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Animals were then placed on a sterile pad and their abdomen and scrotum washed with betadine and 70% alcohol. The testes were removed via a midline scrotal incision with sterile suture being used to ligate supra-testicular tissue prior to surgical removal of each testis. The surgical wound site was closed with sterile stainless steel wound clips and the site cleaned with betadine. The animals were allowed to recover on a sterile pad (until able to stand) and then returned
to their cage. Alzet osmotic pump(s) (model 2002) was placed subcutaneouly in the scapular region. In this instance the scapular region was shaved and cleaned (betadine and alcohol) and a small incision (1 cm) made using a sterile scalpel. The osmotic pump was inserted and the wound closed with a sterile stainless steel wound clip.
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